Research Article

Electrophoretic Separation of Influenza Virus Ribonucleoproteins

Journal of General Virology 1981; 53(1):125 · https://doi.org/10.1099/0022-1317-53-1-125

View at publisher PubMed

Abstract

A new procedure for the separation of influenza virus particle ribonucleoproteins (RNPs) by electrophoresis on a slab of polyacrylamide gel resolves five discrete bands. The largest is a triplet containing the three largest RNAs (1 to 3), the intermediate sized RNP is a doublet containing RNAs 5 and 6 and the others each contain a single RNA. In addition, each RNP is composed of NP protein and an amount of M which is independent of the size of the RNP. This suggests that some virus particle M is specifically associated with influenza ribonucleoprotein.