Abstract
The RNA content of encephalomyocarditis virus was found to be 31.7%, a figure obtained by combining results from nitrogen and phosphorus determinations which gave 32.0%, from extinction measurements at 260 nm. on perchloric acid hydrolysates which gave 31.3% and from orcinol determinations which gave 31.8%. Specific extinction coefficients obtained were E at 260 nm. = 77.4 for the virus in 0.1 M-KCl + 0.02 M-phosphate buffer, pH 8.0, and E at 260 nm. = 221 for its RNA in 0.1 M-tris + HCl, pH 7.2.
* Present address and reprints from: Sloan-Kettering Institute for Cancer Research, New York, N.Y. 10021.