Research Article

Nucleotide Sequence of the Capsid Protein Gene and 3' Non-coding Region of Papaya Mosaic Virus RNA

Journal of General Virology 1988; 69(1):219 · https://doi.org/10.1099/0022-1317-69-1-219

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Abstract

The nucleotide sequences of cDNA clones corresponding to the 3' OH end of papaya mosaic virus RNA have been determined. The 3'-terminal sequence obtained was 900 nucleotides in length, excluding the poly(A) tail, and contained an open reading frame capable of giving rise to a protein of 214 amino acid residues with an Mr of 22930. This protein was identified as the viral capsid protein. The 3' non-coding region of PMV genome RNA was about 121 nucleotides long [excluding the poly(A) tail] and homologous to the complementary sequence of the non-coding region at the 5' end of PMV RNA. A long open reading frame was also found in the predicted 5' end region of the negative strand.