Abstract
The complete nucleotide sequence of the RNA segment 10 of Australian BTV serotype 1 has been deduced from a combination of sequencing cDNA inserts cloned into pBR322 and synthetic deoxynucleotide priming on purified double-stranded RNA molecules. The gene segment was 822 nucleotides in length and capable of coding for two proteins on either 229 or 216 amino acids, having net charges of either +4.5 or +5.5 respectively at neutral pH. Comparison with the RNA segment 10 from BTV serotype 10 revealed a high degree of amino acid sequence conservation as well as regions of nucleic acid sequence conservation.