Abstract
Three serologically distinct bovine enteroviruses (VG-5-27; VC-65-182; VP-7-19) grew in BHK21 cells to concentrations of approximately 5 x 108 p.f.u./ml. A purification procedure was used which involved sedimentation and gel-filtration through a Sepharose 2 B column. The virus particles were spherical (diameter of 27 nm.), had a buoyant density of 1.34 g./ml. in CsCl and a sedimentation coefficient of approximately 165s. The RNA of each of the virus types had a sedimentation coefficient of 35S in sucrose-gradients and the base compositions were similar, being characterized by high A (30%) and low U (21%).