Research Article

Evidence for the Involvement of the 58K and 48K Proteins in the Intercellular Movement of Cowpea Mosaic Virus

Journal of General Virology 1990; 71(1):219 · https://doi.org/10.1099/0022-1317-71-1-219

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Abstract

Infection of cowpea cells with cowpea mosaic virus (CPMV) is accompanied by the appearance of tubular structures containing virus-like particles which protrude from or penetrate the cell wall. Immunogold labelling of sections of infected cells using antisera against a CPMV M RNA translation products, and Protein A-gold, showed that the 58K and/or 48K tentative transport proteins of CPMV were located in or on these tubular structures. Furthermore, these proteins were detected in small electron-dense areas near the tail-end of the tubules. The possible function of these structures in virus movement from cell to cell is discussed.