Nucleotide sequences of normal and rearranged RNA segments 10 of human rotaviruses

Normal and rearranged RNA segments 10 of group A rotaviruses isolated from a chronically infected immunodeficient child were amplified by the polymerase chain reaction as full-length cDNA copies, and were subsequently cloned and sequenced. Compared with the nucleotide sequence of the normal RNA segment 10, the rearranged form contains a partial non-coding duplication at its 3' end and several point mutations. The normal RNA segment 10 was similar to that of bovine rotavirus.

^† Present address: Public Health Laboratory, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2QW, U.K.

The nucleotide sequence data reported in this paper will appear in the DDBJ, EMBL and GenBank nucleotide sequence databases under the accession numbers D01145 and D01146.