Serological and molecular evidence of rhesus papillomavirus type 1 infections in tissues from geographically distinct institutions

The¹ Institute of Human Genetics
and² Department of Microbiology,
³ Laboratory Medicine and Pathology, University of Minnesota Medical School, Minneapolis, MN 55455, USA
⁴ The R. W. Johnson Pharmaceutical Research Institute, Raritan, NJ 08869, USA
⁵ The Tulane Regional Primate Research Center, Covington, LA 70433, USA
⁶ The Wisconsin Regional Primate Research Center, Madison, WI 53715, USA
and⁷ The Fred Hutchinson Cancer Research Center, Seattle, WA 98104, USA

We have previously demonstrated the presence of rhesus monkey papillomavirus type 1 (RhPV-1), from molecular and pathological evidence, in a mating group within a single institution. We have now also obtained a number of fresh or archival tissues of rhesus monkeys from other geographically distinct institutions. Using PCR amplification, we observed two animals from one of these institutions and five animals from another which demonstrated RhPV-1 DNA sequences. In addition we molecularly cloned the E7, E2, E4, L2 and L1 genes of RhPV-1 into bacterial expression vectors. The fusion gene products were used to test for serological response to RhPV-1 antigens by Western blot analysis. Responses were observed in up to 52% of the animals tested. While some serologically positive animals were also RhPV-1 DNA-positive, most were not.

^* Author for correspondence. Fax +1 612 626 7031. e-mail ron@molbio.cbs.umn.edu