Summary auto-generated
This study describes the characterization and formal identification of a new bacterial species, Streptomyces thermospinisporus, isolated from garden soil. The organism is a facultatively carboxydotrophic actinomycete, meaning it can use carbon monoxide or carbon dioxide as sole carbon sources for energy and growth. Using polyphasic taxonomy, researchers examined the strain's morphological, chemical, and genetic properties. The strain formed gray aerial spores with spiny surfaces and grows optimally at 45°C. Phylogenetic analysis based on 16S rRNA gene sequencing showed the strain formed a distinct lineage within the Streptomyces genus, most closely related to S. thermocarboxydovorans but clearly distinguishable. DNA-DNA relatedness studies confirmed it represents a separate genomic species, sharing only 72% relatedness with closely related species, below the 80% threshold for genomic species recognition. The strain's distinct phenotypic properties, including unique carbon source utilization patterns and chemical composition, further differentiate it from related thermophilic Streptomyces species. These combined analyses support the proposal of Streptomyces thermospinisporus as a new species.
Key findings
- Streptomyces thermospinisporus is a facultatively carboxydotrophic, moderately thermophilic actinomycete capable of using carbon monoxide and carbon dioxide as sole carbon sources
- 16S rRNA phylogenetic analysis revealed the strain forms a distinct lineage within Streptomyces, sharing 99.2% similarity with S. thermocarboxydovorans but differing by 11 nucleotides across 1414 positions
- DNA-DNA relatedness of 72% with closest relatives falls below the 80% species recognition threshold, confirming distinct genomic status
- The strain is distinguished by spore chains with spiny ornamentation, gray aerial spore mass, and unique phenotypic properties including selective carbon source utilization
- Strain AT10T (DSM 41779T) was isolated from garden soil and shows optimal growth at 45°C with a genomic GC content of 73.6 mol%
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Abstract
A carboxydotrophic actinomycete strain, AT10(T) (=DSM 41779(T)=KCTC 9909(T)), was the subject of a polyphasic study. The morphological and chemical properties of the strain were found to be consistent with its assignment to the genus Streptomyces. The organism formed a distinct phyletic line within the 16S rDNA Streptomyces tree, and DNA--DNA relatedness experiments further confirmed that it formed a distinct genomic species. The strain was also distinguished from related species using phenotypic properties. Strain AT10(T), therefore, merits species status within the genus Streptomyces; the name Streptomyces thermospinisporus sp. nov. is proposed for this new taxon.