Winogradskyella poriferorum sp. nov., a novel member of the family Flavobacteriaceae isolated from a sponge in the Bahamas

A Gram-negative, rod-shaped bacterium (designated strain UST030701-295^T) with fast gliding motility was isolated from the surface of the sponge Lissodendoryx isodictyalis in the Bahamas. Colonies of UST030701-295^T were yellow in colour, 24 mm in diameter, convex with a smooth surface and entire margins. UST030701-295^T was heterotrophic, strictly aerobic and required NaCl for growth (1·04·0 %). Growth was observed at pH 6·010·0 and at 1244 °C. Phylogenetic analysis of the 16S rRNA gene sequence placed UST030701-295^T within the genus Winogradskyella of the family Flavobacteriaceae, sharing 94·795·8 % similarity with the three recognized members of the genus. The G+C content of the DNA was 32·8 mol% and the predominant fatty acids were iso-C_{15 : 1}, iso-C_{15 : 0}, iso-C_{15 : 0} 2-OH, iso-C_{15 : 0} 3-OH, iso-C_{16 : 0} 3-OH, C_{16 : 1}ω7 and iso-C_{17 : 0} 3-OH (together representing 75·4 % of the total); these data supported the affiliation of UST030701-295^T to the genus Winogradskyella. UST030701-295^T differed from the three recognized species of Winogradskyella in 717 traits. Molecular evidence together with phenotypic characteristics suggests that UST030701-295^T represents a novel species within the genus Winogradskyella, for which the name Winogradskyella poriferorum sp. nov. is proposed. The type strain is UST030701-295^T (=NRRL B-41101^T=JCM 12885^T).