Algimonas ampicilliniresistens sp. nov., isolated from the red alga Porphyra yezoensis, and emended description of the genus Algimonas

Youhei Fukui, Masahiro Kobayashi, Hiroaki Saito, Hiroshi Oikawa, Yutaka Yano, Masataka Satomi

¹National Research Institute of Fisheries Science, Fisheries Research Agency, Yokohama 236-8648, Japan

²Seikai National Fisheries Research Institute, Fisheries Research Agency, Nagasaki 851-2213, Japan

³National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency, Hatsukaichi 739-0452, Japan

⁴Hokkaido National Fisheries Research Institute, Fisheries Research Agency, Sapporo 062-0922, Japan

Correspondence
Youhei Fukui yfukui{at}affrc.go.jp.

International Journal of Systematic and Evolutionary Microbiology 2013; 63(Pt 12):4407–4412 · https://doi.org/10.1099/ijs.0.053405-0

Abstract

Three strains (14A-2-7^T, 14A-3-1 and 14A-3) of Gram-stain-negative, prosthecate, motile bacteria were isolated from an algal medium supplemented with 10 mg ampicillin l⁻¹ (w/v), in which the red alga Porphyra yezoensis had been cultured. Based on the 16S rRNA gene sequence analysis, the three isolates formed a cluster with the genus Algimonas of the family Hyphomonadaceae. The sequences of the three isolates had high similarity with those of Algimonas porphyrae 0C-2-2^T (97.6 % similarity) and Litorimonas taeanensis G5^T (95.6 % similarity). The DNA G+C contents of the three isolates ranged from 54.3 to 55.0 mol%, which were more similar to that of A. porphyrae 0C-2-2^T (58.5 mol%) than to that of L. taeanensis G5^T (47.1 mol%). The DNA–DNA relatedness showed that the three isolates were representatives of the same species (88.1–94.0 % relatedness) and that strain 14A-2-7^T was a representative of a different species from A. porphyrae 0C-2-2^T and L. taeanensis G5^T (1.2–8.6 % relatedness). The phenotypic characteristics of strain 14A-2-7^T differed by 20 results and 30 results from A. porphyrae 0C-2-2^T and L. taeanensis G5^T, respectively. The three isolates contained ubiquinone-10 as the predominant quinone and C_18 : 1ω7c as the major fatty acid. Based on the polyphasic taxonomic analysis, the three isolates represent a novel species of the genus Algimonas, for which the name Algimonas ampicilliniresistens sp. nov. is proposed. The type strain is 14A-2-7^T ( = LMG 26421^T = NBRC 108219^T). An emended description of the genus Algimonas is also proposed.