SGM Journals
Actinobacteria

Alloscardovia macacae sp. nov., isolated from the milk of a macaque (Macaca mulatta), emended description of the genus Alloscardovia and proposal of Alloscardovia criceti comb. nov.

J. Killer, Š. Ročková, E. Vlková, V. Rada, J. Havlík, J. Kopečný, V. Bunešová, O. Benada, O. Kofroňová, R. Pechar, I. Profousová

  • 1Institute of Animal Physiology and Genetics v.v.i., Academy of Sciences of the Czech Republic, Vídeňská 1083, Prague 4 – Krč, 142 20, Czech Republic
  • 2Czech University of Life Sciences, Faculty of Agrobiology, Food and Natural Resources, Department of Microbiology, Nutrition and Dietetics, Kamýcká 129, Prague 6 – Suchdol, 165 21, Czech Republic
  • 3Laboratory of Molecular Structure Characterization, Institute of Microbiology, Institute of Microbiology, v.v.i., Academy of Sciences of the Czech Republic, Vídeňská 1083, Prague 4 – Krč, 142 20, Czech Republic
  • 4Department of Biology, Faculty of Science, J.E. Purkyně University in Ústi nad Labem, Za Válcovnou 1000/8, 400 96 Ústí nad Labem, Czech Republic
  • 5University of Veterinary and Pharmaceutical Sciences, Department of Pathological morphology and Parasitology, Palackého 1/3, Brno 612 42, Czech Republic
  • Correspondence
    J. Killer Killer.Jiri{at}seznam.cz or killer{at}iapg.cas.cz

    • International Journal of Systematic and Evolutionary Microbiology 2013; 63(Pt 12):4439–4446 · https://doi.org/10.1099/ijs.0.051326-0

    View at publisher PubMed

    Abstract

    A novel bacterial strain, designated M8T, was isolated from milk of a female macaque bred in captivity. The strain was Gram-stain-positive, anaerobic, irregular coccoid–rod-shaped without catalase activity. Analysis of 16S rRNA gene sequence similarity revealed that the isolate was most closely related to Alloscardovia omnicolens CCUG 31649T (96.4 %) and Metascardovia criceti OMB105T (96.6 %). Sequences of hsp60, fusA, and xfp genes also confirmed that the strain was most closely related to the type strains of A. omnicolens and M. criceti. The isolate produced fructose-6-phosphate phosphoketolase which is in agreement with classification within the family Bifidobacteriaceae. The major fatty acids were C18 : 1ω9c (35.8 %), C16 : 1 (6.2 %) and C14 : 0 (5.7 %). Polar lipid analysis revealed five different glycolipids, two unidentified phospholipids and diphosphatidylglycerol. The peptidoglycan was of the type A4α l-Lys–d-Asp with the presence of d(l)-alanine, d-glutamine, d-asparagine and l-lysine. The DNA G+C content of strain M8T was 50.1 mol%. On the basis of genetic, phylogenetic and phenotypic data, strain M8T represents a novel species of the genus Alloscardovia for which the name Alloscardovia macacae sp. nov. is proposed. The type strain is M8T ( = DSM 24762T = CCM 7944T). In addition, our results also revealed that Alloscardovia omnicolens DSM 21503T and Metascardovia criceti DSM 17774T do not belong to different genera within the family Bifidobacteriaceae. We therefore propose to reclassify Metascardovia criceti as Alloscardovia criceti comb. nov. An emended description of the genus Alloscardovia is also provided.

    • The GenBank/EMBL/DDBJ accession numbers for the partial 16S rRNA gene, hsp60, Xfp and fusA sequences of strain M8T are JQ363617, JQ363647, JQ363675 and JQ363637, respectively. GenBank/EMBL/DDBJ accession numbers for some of these phylogenetic markers obtained within this study for type strains of A. omnicolens and A. criceti comb. nov. are shown in the phylogenetic trees.

    • Four supplementary figures and three supplementary tables are available with the online version of this paper.

    Abbreviations:
    F6PPK
    fructose-6-phosphate phosphoketolase