Hymenobacter koreensis sp. nov. and Hymenobacter saemangeumensis sp. nov., isolated from estuarine water

Two Gram-reaction-negative, rod-shaped, non-motile and red–pink-pigmented bacterial strains, designated GYR3077^T and GSR0100^T, were isolated from a water sample of the Mangyung estuary enclosed by the Saemangeum Embankment in JEOLlabuk-do, South Korea, and were characterized using a polyphasic approach. 16S rRNA genes of strains GYR3077^T and GSR0100^T exhibited sequence similarities of 95.9 % to Hymenobacter deserti ZLB-3^T and 96.6 % to Hymenobacter soli PB17^T, respectively, and indicated that these isolates belonged to the phylum Bacteroidetes. The major cellular fatty acids present in the two isolates were iso-C_15 : 0, C_16 : 1ω5c, summed feature 4 (iso-C_17 : 1 I and/or anteiso-C_17 : 1 B) and summed feature 3 (C_16 : 1ω7c and/or C_16 : 1ω6c). The major respiratory quinone and polyamine patterns were menaquinone-7 and sym-homospermidine, characteristic of the genus Hymenobacter. Flexirubin-type pigments were absent in both strains. The DNA G+C contents of strains GYR3077^T and GSR0100^T were 60.2 mol% and 61.9 mol%, respectively. The major polar lipid of strains GYR3077^T and GSR0100^T was phosphatidylethanolamine. Based on the morphological and physiological properties, strains GYR3077^T and GSR0100^T were considered to represent two novel species of the genus Hymenobacter, for which the names Hymenobacter koreensis sp. nov. (type strain GYR3077^T = KACC 16451^T = JCM 17924^T) and Hymenobacter saemangeumensis sp. nov. (type strain GSR0100^T = KACC 16452^T = JCM 17923^T) are proposed.