SGM Journals
Firmicutes And Related Organisms

Lactobacillus silagei sp. nov., isolated from orchardgrass silage

Masanori Tohno, Maki Kitahara, Tomohiro Irisawa, Takaharu Masuda, Ryuichi Uegaki, Moriya Ohkuma, Kiyoshi Tajima

  • 1National Agriculture and Food Research Organization, National Institute of Livestock and Grassland Science, Nasushiobara 329-2793, Japan
  • 2Japan Collection of Microorganisms, RIKEN BioResource Center, Tsukuba, Ibaraki 305-0074, Japan
  • 3Iwate Agricultural Research Center Animal Industry Research Institute, Morioka 028-2711, Japan
  • 4The United Graduate School of Agricultural Sciences, Iwate University, Morioka 020-8550, Japan
  • 5National Agriculture and Food Research Organization, National Institute of Livestock and Grassland Science, Tsukuba, Ibaraki 305-0901, Japan
  • Correspondence
    Masanori Tohno tohno{at}affrc.go.jp

    • International Journal of Systematic and Evolutionary Microbiology 2013; 63(Pt 12):4613–4618 · https://doi.org/10.1099/ijs.0.053124-0

    View at publisher PubMed

    Abstract

    A Gram-reaction-positive, facultatively anaerobic, non-spore-forming and catalase-negative rod-shaped bacterial strain, designated IWT126T, was isolated from orchardgrass (Dactylis glomerata L.) silage preserved in Hachimantai, Iwate, Japan. The isolate showed growth at 15–45 °C, pH 3.5–7.5 and with 4.0 % (w/v) NaCl. The cell wall peptidoglycan did not contain meso-diaminopimelic acid, and the DNA G+C content was 45.6 mol%. The major cellular fatty acids were C16 : 0 and C19 : 1 cyclo 9,10. Based on 16S rRNA gene sequence similarity, strain IWT126T was classified as a member of the genus Lactobacillus and was most closely related to Lactobacillus odoratitofui YIT 11304T (98.7 %), Lactobacillus similis JCM 2765T (98.5 %), Lactobacillus collinoides JCM 1123T (97.6 %), Lactobacillus paracollinoides DSM 15502T (97.6 %) and Lactobacillus kimchicus DCY51T (96.9 %). Based on sequence analysis of the phenylalanyl-tRNA synthase α-subunit (pheS) gene, strain IWT126T was well separated from its phylogenetic neighbours in the genus Lactobacillus. Based on physiological, biochemical and genotypic results, as well as low DNA–DNA relatedness to recognized phylogenetic relatives in the genus Lactobacillus, classification of strain IWT126T as a representive of a novel species named Lactobacillus silagei sp. nov. is proposed. The type strain is IWT126T ( = JCM 19001T = DSM 27022T).

    • The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA and pheS gene sequences of strain IWT126T are AB786910 and AB794992, respectively.

    • Three supplementary figures and a supplementary table are available with the online version of this paper.