SGM Journals
Bacteroidetes

DNA–DNA hybridization study of strains of Chryseobacterium, Elizabethkingia and Empedobacter and of other usually indole-producing non-fermenters of CDC groups IIc, IIe, IIh and IIi, mostly from human clinical sources, and proposals of Chryseobacterium bernardetii sp. nov., Chryseobacterium carnis sp. nov., Chryseobacterium lactis sp. nov., Chryseobacterium nakagawai sp. nov. and Chryseobacterium taklimakanense comb. nov.

B. Holmes, A. G. Steigerwalt, A. C. Nicholson

  • 1National Collection of Type Cultures, Health Protection Agency, Colindale, London NW9 5EQ, UK
  • 2Special Bacteriology Reference Laboratory, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
  • Correspondence
    A. C. Nicholson agn0{at}cdc.gov

    • International Journal of Systematic and Evolutionary Microbiology 2013; 63(Pt 12):4639–4662 · https://doi.org/10.1099/ijs.0.054353-0

    View at publisher PubMed

    Abstract

    The taxonomic classification of 182 phenotypically similar isolates was evaluated using DNA–DNA hybridization and 16S rRNA gene sequence analysis. These bacterial isolates were mainly derived from clinical sources; all were Gram-negative non-fermenters and most were indole-producing. Phenotypically, they resembled species from the genera Chryseobacterium, Elizabethkingia or Empedobacter or belonged to CDC groups IIc, IIe, IIh and IIi. Based on these analyses, four novel species are described: Chryseobacterium bernardetii sp. nov. (type strain NCTC 13530T = CCUG 60564T = CDC G229T), Chryseobacterium carnis sp. nov. (type strain NCTC 13525T = CCUG 60559T = CDC G81T), Chryseobacterium lactis sp. nov. (type strain NCTC 11390T = CCUG 60566T = CDC KC1864T) and Chryseobacterium nakagawai sp. nov. (type strain NCTC 13529T = CCUG 60563T = CDC G41T). The new combination Chryseobacterium taklimakanense comb. nov. (type strain NCTC 13490T = X-65T = CCTCC AB 208154T = NRRL B-51322T) is also proposed to accommodate the reclassified Planobacterium taklimakanense.

    • The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of C. anthropi NCTC 13528, C. bernardetii sp. nov. NCTC 13530T, C. carnis sp. nov. NCTC 13525T, C. gleum-like species 1 strain NCTC 13470, C. haifense NCTC 13466T, C. hominis CDC F5649, C. lactis sp. nov. NCTC 11390T, C. nakagawai sp. nov. NCTC 13529T, C. shigense strains NCTC 13533 and NCTC 13458T, C. taklimakanense comb. nov. NCTC 13527, C. ureilyticum NCTC 13524, F. granuli NCTC 13460T, F. lindanitolerans NCTC 13531, S. daejeonense strains NCTC 13534 and NCTC 13455T, S. lactis NCTC 13526T and W. falsenii NCTC 11310 are respectively JX100815–JX100832.

    • A supplementary table and a supplementary figure are available with the online version of this paper.