SGM Journals
Actinobacteria

Mycobacterium bourgelatii sp. nov., a rapidly growing, non-chromogenic species isolated from the lymph nodes of cattle

Véronique Guérin-Faublée, Jean-Pierre Flandrois, Catherine Pichat, Maria Laura Boschiroli, Brigitte Lamy

  • 1VetAgro Sup Campus Vétérinaire de Lyon, Département de Santé Publique Vétérinaire, Marcy l’Étoile, France
  • 2Université de Lyon, Université Lyon 1, CNRS UMR5558, Laboratoire de Biométrie et Biologie Evolutive, Villeurbanne, France
  • 3Hospices Civils de Lyon, Centre Hospitalier Lyon Sud, Laboratoire de Bactériologie, Pierre Bénite, France
  • 4Agence Nationale de Sécurité Sanitaire de l’Alimentation, du Travail et de l’Environnement, Laboratoire de Santé Animale de Maisons-Alfort, Unité Zoonoses Bactériennes, Maisons-Alfort, France
  • 5Centre Hospitalier de Montpellier, Hôpital Arnaud de Villeneuve, Laboratoire de Bactériologie, Montpellier, France
  • 6Université Montpellier 1, CNRS UMR 5119, Equipe Pathogènes et Environnements, Montpellier, France
  • Correspondence
    Véronique Guérin-Faublée veronique.guerinfaublee{at}vetagro-sup.fr

    • International Journal of Systematic and Evolutionary Microbiology 2013; 63(Pt 12):4669–4674 · https://doi.org/10.1099/ijs.0.051979-0

    View at publisher PubMed

    Abstract

    Three independent strains of a rapidly growing, non-chromogenic member of the genus Mycobacterium were isolated from lymph nodes of French cattle. Identification of the isolates was carried out using a polyphasic approach. The nearly complete SSU rRNA gene sequences (>1200 bp) of the strains MLB-A23, MLB-A30 and MLB-A84T were identical. A phylogenetic analysis of these unique SSU rRNA gene sequences showed that these strains were most closely related to Mycobacterium intermedium. Further phylogenetic analysis based on concatenated sequences (2854 bp) of four housekeeping genes (hsp65, rpoB, sodA and tuf), the transfer–messenger RNA (tmRNA) and SSU rRNA genes indicated that these three strains represented a distinct species that shares a common ancestor with M. intermedium. Phylogenetic and phenotypic data strongly indicate that the strains MLB-A23, MLB-A30 and MLB-A84T belong to a novel mycobacterial species for which the name Mycobacterium bourgelatii sp. nov. is proposed. The type strain is MLB-A84T ( = CIP 110557T = DSM 45746T).

    • The GenBank/EMBL/DDBJ accession numbers for the nearly complete SSU rRNA, partial rpoB, partial sodA, partial hsp65 (groEL2), partial tuf and tmRNA gene sequences of strains MLB-A23, MLB-A30 and MLB-A84T are HF674382–HF674384, HF566388–HF566390, HF585625–HF585627, HF566383–HF566385, HF585628–HF585630 and HF674385–HF674387, respectively.

    • A supplementary table is available with the online version of this paper.

    Abbreviations:
    CFA
    cellular fatty acids
    ML
    maximum-likelihood
    NJ
    neighbour-joining
    NTM
    non-tuberculous mycobacteria