SGM Journals
Firmicutes And Related Organisms

Enterococcus saccharolyticus subsp. taiwanensis subsp. nov., isolated from broccoli

Yi-sheng Chen, Yu-hsuan Lin, Shwu-fen Pan, Si-hua Ji, Yu-chung Chang, Chi-rong Yu, Min-shiuan Liou, Hui-chung Wu, Misa Otoguro, Fujitoshi Yanagida, Chen-chung Liao, Chi-ming Chiu, Bi-qiang Huang

  • 1Department of Biotechnology, Ming Chuan University, No. 5, De-Ming Road, Gui-Shan Township, Taoyuan County 333, Taiwan
  • 2The Institute of Enology and Viticulture, Yamanashi University, 1-13-1, Kitashin, Kofu, Yamanashi 400-0005, Japan
  • 3Proteomics Research Center, National Yang-Ming University, Taipei 11221, Taiwan
  • Correspondence
    Yi-sheng Chen yisheng{at}mail.mcu.edu.tw

    • International Journal of Systematic and Evolutionary Microbiology 2013; 63(Pt 12):4691–4697 · https://doi.org/10.1099/ijs.0.052761-0

    View at publisher PubMed

    Abstract

    A coccal strain isolated from fresh broccoli was initially identified as Enterococcus saccharolyticus; however, molecular identification and phenotypic traits did not support this identification. DNA–DNA hybridization with the type strain of E. saccharolyticus (76.4 % relatedness), DNA G+C content (35.7 mol%), phylogenetic analysis based on 16S rRNA, pheS and rpoA gene sequences, rep-PCR fingerprinting and profiles of cellular fatty acids, whole-cell proteins and enzyme activities, together with carbohydrate metabolism characteristics, indicated that this strain is distinct and represents a novel subspecies, for which the name Enterococcus saccharolyticus subsp. taiwanensis subsp. nov. is proposed. The type strain is 812T ( = NBRC 109476T = BCRC 80575T). Furthermore, we present an emended description of Enterococcus saccharolyticus and proposal of Enterococcus saccharolyticus subsp. saccharolyticus subsp. nov. (type strain ATCC 43076T = CCUG 27643T = CCUG 33311T = CIP 103246T = DSM 20726T = JCM 8734T = LMG 11427T = NBRC 100493T = NCIMB 702594T).

    • The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA, pheS and rpoA gene sequences of strain 812T are AB775175–AB775177, respectively.

    • Nine supplementary figures and a supplementary table are available with the online version of this paper.