SGM Journals
Proteobacteria

Burkholderia humi sp. nov., Burkholderia choica sp. nov., Burkholderia telluris sp. nov., Burkholderia terrestris sp. nov. and Burkholderia udeis sp. nov.: Burkholderia glathei-like bacteria from soil and rhizosphere soil

Peter Vandamme, Evie De Brandt, Kurt Houf, Joana Falcão Salles, Jan Dirk van Elsas, Theodore Spilker, John J. LiPuma

  • 1Laboratorium voor Microbiologie, Vakgroep Biochemie en microbiologie, Faculteit Wetenschappen, Universiteit Gent, Gent, Belgium
  • 2Vakgroep Veterinaire Volksgezondheid en Voedselveiligheid, Faculteit Diergeneeskunde, Universiteit Gent, Gent, Belgium
  • 3Department of Microbial Ecology, Center for Life Sciences, University of Groningen, The Netherlands
  • 4Department of Pediatrics and Communicable Disease, University of Michigan Medical School, Ann Arbor, MI 48109, USA
  • Correspondence
    Peter Vandamme Peter.Vandamme{at}ugent.be

    • International Journal of Systematic and Evolutionary Microbiology 2013; 63(Pt 12):4707–4718 · https://doi.org/10.1099/ijs.0.048900-0

    View at publisher PubMed

    Abstract

    Analysis of partial gyrB gene sequences revealed six taxa in a group of 17 Burkholderia glathei-like isolates which were further examined by (GTG)5-PCR fingerprinting, 16S rRNA gene sequence analysis, DNA–DNA hybridizations, determination of the DNA G+C content, whole-cell fatty acid analysis and an analysis of cell and colony morphology and more than 180 biochemical characteristics. The results demonstrated that one taxon consisting of three human clinical isolates represented Burkholderia zhejiangensis, a recently described methyl-parathion-degrading bacterium isolated from a wastewater-treatment system in China. The remaining taxa represented five novel species isolated from soil or rhizosphere soil samples, and could be distinguished by both genotypic and phenotypic characteristics. We therefore propose to formally classify these bacteria as Burkholderia humi sp. nov. (type strain, LMG 22934T = CCUG 63059T), Burkholderia choica sp. nov. (type strain, LMG 22940T = CCUG 63063T), Burkholderia telluris sp. nov. (type strain, LMG 22936T = CCUG 63060T), Burkholderia udeis sp. nov. (type strain, LMG 27134T = CCUG 63061T) and Burkholderia terrestris sp. nov. (type strain, LMG 22937T = CCUG 63062T).

    • The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequence of strains LMG 22936T and LMG 22937T, and Burkholderia zhejiangensis LMG 26180, LMG 20181 and LMG 27249 are HE981727, HE981726, HE983367, HE983368 and HE983369, respectively. The GenBank/EMBL/DDBJ accession numbers for the gyrB sequences determined in this study are HE985160–HE985179, HF545445–HF545447 and HF547269 (see Fig. 1 for strain details).

    • A supplementary table and a supplementary figure are available with the online version of this paper.

    Abbreviations:
    FAME
    fatty acid methyl ester
    UPGMA
    unweighted pair group using arithmetic means