Zhihengliuella flava sp. nov., an actinobacterium isolated from sea sediment, and emended description of the genus Zhihengliuella

Moriyuki Hamada, Chiyo Shibata, Tomohiko Tamura, Ken-ichiro Suzuki

Biological Resource Center, National Institute of Technology and Evaluation (NBRC) 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan

Correspondence
Moriyuki Hamada hamada-moriyuki{at}nite.go.jp

International Journal of Systematic and Evolutionary Microbiology 2013; 63(Pt 12):4760–4764 · https://doi.org/10.1099/ijs.0.053272-0

Abstract

A novel Gram-staining-positive actinobacterium, designated H85-3^T, was isolated from a sea sediment sample and its taxonomic position was investigated by a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain H85-3^T was closely related to the members of the genus Zhihengliuella with pairwise sequence similarities of 97.4–98.6 %. The peptidoglycan of strain H85-3^T was found to be of the A4α type with lysine as the diagnostic diamino acid. The menaquinones were MK-9, MK-10 and MK-8 (56 : 30 : 14) and the major cellular fatty acids were anteiso-C_15 : 0, iso-C_15 : 0 and anteiso-C_17 : 0. These data supported the affiliation of strain H85-3^T to the genus Zhihengliuella. Meanwhile, the results of DNA–DNA hybridization, along with the differences in some phenotypic characteristics, indicated that strain H85-3^T should be distinguished from the recognized species of the genus Zhihengliuella. Therefore, strain H85-3^T represents a novel species of the genus Zhihengliuella, for which the name Zhihengliuella flava sp. nov. is proposed; the type strain is H85-3^T ( = NBRC 109021^T = DSM 26152^T). An emended description of the genus Zhihengliuella is also proposed.