Chryseobacterium angstadtii sp. nov., isolated from a newt tank

As part of an undergraduate microbiology course, a yellow–orange-pigmented, Gram-staining negative, rod-shaped, non-motile bacterial strain was isolated from a glass tank housing several red-spotted newts (Notophthalmus viridescens). The sequence of the 16S rRNA gene of this strain, designated KM^T, was 97.4–98.0 % similar to those of the type strains of Chryseobacterium luteum, C. shigense and C. vrystaatense, while the similarity levels for protein-coding genes were less than 94.7 % for rpoB, less than 92.1 % for groEL and less than 87.1 % for gyrB. These values are lower than for many other established distinct species. Polyphasic characterization and comparison to these relatives revealed that strain KM^T was similar to other Chryseobacterium strains in that it contained MK-6 as its major respiratory quinone and phosphatidylethanolamine as the most abundant polar lipid, produced flexirubin-type pigments, oxidase and catalase and primarily contained the fatty acids iso-C_15 : 0, iso-C_17 : 1ω9c, iso-C_17 : 0 3-OH and summed feature 3 (comprising C_16 : 1ω6c and/or C_16 : 1ω7c). Based on the results of this study, strain KM^T represents a novel species, for which the name Chryseobacterium angstadtii sp. nov. is proposed. The type strain is KM^T ( = ATCC BAA-2160^T = NRRL B-59516^T = KCTC 23297^T).