Kordia aquimaris sp. nov., a zeaxanthin-producing member of the family Flavobacteriaceae isolated from surface seawater, and emended description of the genus Kordia

Asif Hameed, Mariyam Shahina, Shih-Yao Lin, Jang-Cheon Cho, Wei-An Lai, Chiu-Chung Young

¹Department of Soil & Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung 402, Taiwan

²Division of Biology and Ocean Sciences, Inha University, Incheon 402-751, Republic of Korea

³Agricultural Biotechnology Center, National Chung Hsing University, Taichung 402, Taiwan

Correspondence
Chiu-Chung Young ccyoung{at}mail.nchu.edu.tw

International Journal of Systematic and Evolutionary Microbiology 2013; 63(Pt 12):4790–4796 · https://doi.org/10.1099/ijs.0.056051-0

Abstract

A Gram-staining-negative, strictly aerobic, rod-shaped, non-flagellated, non-spore-forming and gliding marine bacterium designated strain CC-AMZ-301^T was isolated from coastal surface seawater near Taichung harbour, Taiwan. Strain CC-AMZ-301^T predominantly synthesized zeaxanthin and thus formed yellow colonies on marine agar. The novel strain showed high pairwise 16S rRNA gene sequence similarity to Kordia periserrulae IMCC1412^T (95.0 %), K. algicida KCTC 8814P^T (94.4 %) and K. antarctica IMCC3317^T (94.2 %), and formed a phylogenetic lineage tightly associated with species of the genus Kordia. The polar lipid profile of strain CC-AMZ-301^T consisted of three unidentified lipids, three unidentified aminolipids and one unidentified phospholipid. The major fatty acids (>5 % of total) were iso-C_17 : 0 3-OH, iso-C_15 : 0, summed feature 3 (C_16 : 1ω7c and/or C_16 : 1ω6c) and summed feature 9 (iso-C_17 : 1ω9c and/or 10-methyl C_16 : 0). The DNA G+C content was 36.4 mol%. Menaquinone 6 (MK-6) was the predominant respiratory quinone. The major polyamine was the triamine sym-homospermidine. Based on evidence from the present polyphasic study, strain CC-AMZ-301^T is proposed to represent a novel species of the genus Kordia, for which the name Kordia aquimaris sp. nov. is proposed; the type strain is CC-AMZ-301^T ( = JCM 18556^T = BCRC 80464^T). An emended description of the genus Kordia is also proposed.