SGM Journals
Bacteroidetes

Sphingobacterium thermophilum sp. nov., of the phylum Bacteroidetes, isolated from compost

Shuhei Yabe, Yoshifumi Aiba, Yasuteru Sakai, Masaru Hazaka, Kazuyoshi Kawahara, Akira Yokota

  • 1Hazaka Plant Research Center, Kennan Eisei Kogyo Co., Ltd, 44 Aza-Inariyama, Oaza-Ashitate, Murata-cho, Shibata-gun, Miyagi 989-1311, Japan
  • 2Department of Applied Material and Life Science, College of Engineering, Kanto Gakuin University, Rokkurahigashi, Kanazawa-ku, Yokohama 236-8501, Japan
  • 3Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi Bunkyo-ku, Tokyo 113-0032, Japan
  • Correspondence
    Shuhei Yabe kennan-6{at}kennan-e.co.jp

    • International Journal of Systematic and Evolutionary Microbiology 2013; 63(Pt 5):1584–1588 · https://doi.org/10.1099/ijs.0.042481-0

    View at publisher PubMed

    Abstract

    A Gram-negative bacterium, designated CKTN2T, was isolated from compost. Cells of strain CKTN2T were strictly aerobic rods. The isolate grew at 20–50 °C (optimum 40–45 °C), but not below 15 °C or above 52 °C, and at pH 5.9–8.8 (optimum pH 7.0), but not below pH 5.4 or above pH 9.3. The DNA G+C content was 40.3 mol%. The predominant menaquinone was MK-7. The major fatty acids were iso-C15 : 0 (45.2 %), iso-C17 : 0 3-OH (11.1 %) and C18 : 0 (14.5 %). Analysis of the 16S rRNA gene sequence of strain CKTN2T revealed that it is a member of the genus Sphingobacterium and is most closely related to Sphingobacterium alimentarium DSM 22362T (93.2 % 16S rRNA gene sequence similarity). Strain CKTN2T could be distinguished from its closest phylogenetic relatives by different phenotypic characteristics. According to the phenotypic and genotypic characteristics, strain CKTN2T represents a novel species of the genus Sphingobacterium, for which the name Sphingobacterium thermophilum sp. nov. is proposed. The type strain is CKTN2T ( = JCM 17858T  = KCTC 23708T).

    • The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain CKTN2T is AB563783.