SGM Journals
Proteobacteria

Xenorhabdus ishibashii sp. nov., isolated from the entomopathogenic nematode Steinernema aciari

Ryusei Kuwata, Li-hong Qiu, Wen Wang, Yuki Harada, Mutsuhiro Yoshida, Eizo Kondo, Toyoshi Yoshiga

  • 1Laboratory of Nematology, Faculty of Agriculture, Saga University, 1 Honjo, Saga, Saga 840-8502, Japan
  • 2State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, PR China
  • 3Agro-Environment Research Division, Kyushu Okinawa Agricultural Research Center, 2421 Suya, Koshi, Kumamoto 861-1192, Japan
  • Correspondence
    Ryusei Kuwata ryusei{at}nih.go.jp

    • International Journal of Systematic and Evolutionary Microbiology 2013; 63(Pt 5):1690–1695 · https://doi.org/10.1099/ijs.0.041145-0

    View at publisher PubMed

    Abstract

    Gram-negative bacteria of the genus Xenorhabdus exhibit a mutualistic association with steinernematid entomopathogenic nematodes and a pathogenic relationship with insects. Here we describe two isolates of the entomopathogenic nematode Steinernema aciari collected from China and Japan. 16S rRNA gene sequence similarity and phylogenetic analysis indicated that the isolates obtained from S. aciari belonged to the genus Xenorhabdus. Multilocus sequence analysis based on five universal protein-coding gene sequences revealed that the isolates were closely related to Xenorhabdus ehlersii DSM 16337T and Xenorhabdus griffiniae ID10T but that they exhibited <97 % sequence similarity with these reference strains, which indicated that the isolates were distinct from previously described species. Based on these genetic differences and several differential phenotypic traits, we propose that the isolates represent a novel species of the genus Xenorhabdus, for which we propose the name Xenorhabdus ishibashii sp. nov. The type strain is GDh7T ( = DSM 22670T  = CGMCC 1.9166T).

    • ↵† These authors contributed equally to this work.

    • The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene, recA, gyrB, dnaN, gltX and infB sequences of strain GDh7T are GQ149086 and JQ348906–JQ348910 and of strain IkWj136 are AB243427, AB630947–AB630950 and AB690831, respectively.

    • A supplementary figure is available with the online version of this paper.