SGM Journals
Firmicutes And Related Organisms

Enterococcus xiangfangensis sp. nov., isolated from Chinese pickle

Chun Yan Li, Fen Tian, Ya Dong Zhao, Chun Tao Gu

  • 1Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, PR China
  • 2Synergetic Innovation Center of Food Safety and Nutrition, Northeast Agricultural University, Harbin 150030, PR China
  • Correspondence
    Chun Tao Gu ctgu1977{at}hotmail.com

    • International Journal of Systematic and Evolutionary Microbiology 2014; 64(Pt 3):1012–1017 · https://doi.org/10.1099/ijs.0.058917-0

    View at publisher PubMed

    Abstract

    A Gram-stain-positive bacterial strain, 11097T, was isolated from traditional pickle in Heilongjiang Province, China. The bacterium was characterized using a polyphasic approach, including 16S rRNA gene sequence analysis, phenylalanyl-tRNA synthase (pheS) gene sequence analysis, RNA polymerase α subunit (rpoA) gene sequence analysis, fatty acid methyl ester (FAME) analysis, determination of DNA G+C content, DNA–DNA hybridization and an analysis of phenotypic features. Strain 11097T was phylogenetically related to Enterococcus devriesei, E. pseudoavium, E. viikkiensis, E. avium, E. malodoratus, E. gilvus and E. raffinosus. Strain 11097T had 99.1–99.9 % 16S rRNA gene sequence similarities, 78.2–83.2 % pheS gene sequence similarities and 93.8–96.6 % rpoA gene sequence similarities with type strains of phylogenetically related species. Based upon polyphasic characterization data obtained in the present study, a novel species of the genus Enterococcus, Enterococcus xiangfangensis sp. nov., is proposed with the type strain 11097T ( = LMG 27495T = NCIMB 14834T).

    • The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA, pheS and rpoA gene sequences of strain 11097T are HF679036, HF679043 and HF679047, respectively.

    • Three supplementary figures are available with the online version of this paper.

    Abbreviations:
    FAME
    fatty acid methyl ester