SGM Journals
Firmicutes And Related Organisms

Vagococcus entomophilus sp. nov., from the digestive tract of a wasp (Vespula vulgaris)

J. Killer, P. Švec, I. Sedláček, J. Černohlávková, O. Benada, Z. Hroncová, J. Havlík, E. Vlková, V. Rada, J. Kopečný, O. Kofroňová

  • 1Institute of Animal Physiology and Genetics v.v.i., Academy of Sciences of the Czech Republic, Vídeňská 1083, Prague 4 – Krč, 142 20, Czech Republic
  • 2Czech University of Life Sciences, Faculty of Agrobiology, Food and Natural Resources, Department of Microbiology, Nutrition and Dietetics, Kamýcká 129, Prague 6 – Suchdol, 165 21, Czech Republic
  • 3Czech Collection of Microorganisms, Department of Experimental Biology, Faculty of Science, Masaryk University, Tvrdého 14, 60200 Brno, Czech Republic
  • 4Laboratory of Molecular Structure Characterization, Institute of Microbiology, Institute of Microbiology, v.v.i., Academy of Sciences of the Czech Republic, Vídeňská 1083, Prague 4 – Krč, 142 20, Czech Republic
  • 5Department of Biology, Faculty of Science, J.E. Purkyně University in Ústi nad Labem, Za Válcovnou 1000/8, 400 96 Ústí nad Labem, Czech Republic
  • Correspondence
    J. Killer Killer.Jiri{at}seznam.cz or killer{at}iapg.cas.cz

    • International Journal of Systematic and Evolutionary Microbiology 2014; 64(Pt 3):731–737 · https://doi.org/10.1099/ijs.0.054940-0

    View at publisher PubMed

    Abstract

    Three unknown Gram-stain-positive, catalase-negative, facultatively anaerobic and coccus-shaped strains of bacteria were isolated from the digestive tracts of wasps (Vespula vulgaris). Analysis of 16S rRNA gene sequences revealed that these strains had identical sequences and showed that Vagococcus salmoninarum, with 96.2 % sequence similarity, was the closest phylogenetic neighbour. Further analyses based on hsp60 and pheS gene sequences of representatives of the family Enteroccocaceae and genotypic and phenotypic characterization using (GTG)5-PCR fingerprintings, EcoRI ribotyping, DNA G+C content, whole-cell protein profiling, cellular fatty acid profiles analysis and extensive biotyping confirmed that the investigated strains were representatives of a novel bacterial species within the genus Vagoccocus for which the name Vagoccocus entomophilus sp. nov. is proposed. The type strain is VOSTP2T ( = DSM 24756T = CCM 7946T).

    • Vagococcus entomophilus sp. nov.

    • The GenBank/EMBL/DDBJ accession numbers for the partial 16S rRNA, hsp60, pheS, rpoA, atpA gene sequences and ITS (Internal Transcribed Spacer) sequences for strains VOSTP2T and VOSTP6 are JQ363620–JQ363621, JQ363682–JQ363683, JQ363691–JQ363692, JQ363699–JQ363700, JQ363706–JQ363707 and JQ363708–JQ363709, respectively.

    • Four supplementary figures and three supplementary tables are available with the online version of this paper.