Abstract
SUMMARY: The production of trichothecin by strains of Trichothecium roseum was greatly increased wnen nutrients such as yeast extract, malt extract or corn-steep liquor were added to Czapek-Dox medium containing 5 % (w/v) glucose. Ammonium salts, especially ammonium tartrate, were better than nitrate as source of nitrogen, and high trichothecin titres were also obtained with asparagine or glycine as source of nitrogen. Maximum yields of the order of 100 mg./l. were obtained on a modified Czapek-Dox medium containing ammonium tartrate instead of sodium nitrate and including 5 % (w/v) glucose and 1 % (v/v) of corn-steep liquor, in about 30 days, with 4 cm. depth of medium.