Research Article

Rapid Purification of Lymphocytic Choriomeningitis Virus by Density Gradient Centrifugation in Colloidal Silica

Journal of General Virology 1979; 42(1):215 · https://doi.org/10.1099/0022-1317-42-1-215

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Abstract

Lymphocytic choriomeningitis virus was purified from cell culture fluid by density gradient centrifugation in colloidal silica. The specific infectivity increased 5000-fold and the recovery of infectivity was about 10%.

* Present address: Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie an der Universität Hamburg, Martinistrasse 52, 2000 Hamburg 20, Federal Republic of Germany.