Summary auto-generated
This study completes the genome sequence of Newcastle disease virus (NDV) strain Beaudette C, a highly virulent pathogen affecting all bird species. Researchers determined the nucleotide sequences of the nucleocapsid protein (NP) gene (1747 nucleotides encoding 489 amino acids), the intergenic regions between NP-phosphoprotein, phosphoprotein-matrix protein, and matrix protein-fusion glycoprotein junctions (each 1 nucleotide long), and the 5' trailer region (113 nucleotides). Comparison with the nonvirulent strain B1 showed high conservation of terminal leader and trailer regions, suggesting these elements do not determine virulence. The complete NDV genome is 15,186 nucleotides. Gene junction sequences varied considerably in length (1-47 nucleotides) without sequence identity. The trailer region shared limited identity with other paramyxoviruses but showed structural complementarity suggesting roles in antigenome synthesis. This completed sequence enables reverse genetic approaches to rescue infectious NDV from cloned cDNA, facilitating molecular studies and vaccine development for this important veterinary pathogen.
Key findings
- The complete NDV Beaudette C genome sequence is 15,186 nucleotides, with the NP gene being 1,747 nucleotides encoding 489 amino acids
- Intergenic regions in NDV vary from 1 to 47 nucleotides in length with no conserved sequence identity, unlike other paramyxoviruses
- The 5' trailer region is 113 nucleotides long, intermediate in length between other paramyxoviruses
- Terminal leader and trailer regions are highly conserved between virulent Beaudette C and nonvirulent B1 strains, indicating they do not determine virulence
- Completed genome sequence enables development of reverse genetic systems for rescuing infectious NDV from cDNA
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Abstract
The nucleotide sequences of the nucleocapsid protein (NP) gene, the intergenic regions in the nucleocapsid protein (NP)-phosphoprotein (P), P-matrix protein (M) and M-fusion glycoprotein gene junctions and the trailer region of a virulent Newcastle disease virus (NDV) strain Beaudette C were determined. The NP gene is 1747 nt long and encodes a protein of 489 amino acids. Each of the intergenic sequences determined is 1 nt long and, including the previously published intergenic sequences, the gene junction sequences varied in length from 1-47 nt and lacked any sequence identity. The 5' trailer region is 113 nt in length. Comparison of the sequences of the terminal leader and trailer regions of Beaudette C strain with those of nonvirulent strain B1 showed a high level of conservation, indicating the likelihood of these elements not being a factor in virulence. Together with previously published data, this report completes the sequence of the 15,186 nt genomic RNA of NDV strain Beaudette C.