Research Article

Specific Removal of Host-cell or Vaccinia-virus Antigens from Extracts of Infected Cells by Polyvalent Disulphide-linked Immunosorbents

Journal of General Virology 1970; 8(2):133 · https://doi.org/10.1099/0022-1317-8-2-133

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Abstract

Disulphide-linked immunosorbents prepared from rabbit antisera against extracts of uninfected HeLa (ERK) cells specifically removed host-cell antigens from extracts of cells which had been infected with vaccinia virus. Similarly, disulphide-linked immunosorbents prepared from rabbit anti-vaccinia sera specifically removed vaccinia-specific antigens from infected cell extracts. Disulphide-linked preparations derived from unrelated sera adsorbed neither host nor virus-specific antigens.