SGM Journals
Proteobacteria

Methylobacterium gnaphalii sp. nov., isolated from leaves of Gnaphalium spicatum

Akio Tani, Nurettin Sahin, Kazuhide Kimbara

  • 1Institute of Plant Science and Resources, Okayama University, Chuo 2-20-1, Kurashiki, 710-0046 Okayama, Japan
  • 2Egitim Fakultesi, Mugla University, Kötekli 48000, Mugla, Turkey
  • 3Faculty of Engineering, Shizuoka University, Shizuoka 422-8529, Japan
  • Correspondence
    Akio Tani atani{at}rib.okayama-u.ac.jp

    • International Journal of Systematic and Evolutionary Microbiology 2012; 62(Pt 11):2602–2607 · https://doi.org/10.1099/ijs.0.037713-0

    View at publisher PubMed

    Abstract

    A pink-pigmented, facultatively methylotrophic bacterium, strain 23eT, was isolated from the leaves of Gnaphalium spicatum (cudweed). The cells of strain 23eT were Gram-reaction negative, motile and non-spore-forming rods. On the basis of 16S rRNA gene sequence similarities, strain 23eT was related to Methylobacterium organophilum ATCC 27886T (97.1 %) and Methylobacterium marchantiae JT1T (97 %), and the phylogenetic similarities to all other Methylobacterium species with validly published names were less than 97 %. Major cellular fatty acids were C18 : 1ω7c, C16 : 00 and C18 : 0. The results of DNA–DNA hybridization, phylogenetic analyses based on 16S rRNA and cpn60 gene sequences, fatty acid profiles, whole-cell matrix-assisted laser desorption/ionization time of flight/MS analysis, physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 23eT from the phylogenetically closest relatives. We propose that strain 23eT represents a novel species within the genus Methylobacterium, for which the name Methylobacterium gnaphalii sp. nov. is proposed. The type strain is 23eT ( = DSM 24027T = NBRC 107716T).

    • The GenBank/EMBL/DDBJ accession numbers for the nucleotide sequences of strain 23eT 16S rRNA and partial cpn60 genes are AB627071 and AB627072, of Methylobacterium marchantiae JT1T for the partial cpn60 gene is AB627073, and that of Methylobacterium organophilum ATCC 27886T for the partial cpn60 gene is AB627074.

    • Three supplementary figures are available with the online version of this paper.

    Abbreviations:
    MALDI-TOF
    matrix-assisted laser desorption/ionization time of flight
    PQQ
    pyrroloquinoline quinone