Pseudomonas zeshuii sp. nov., isolated from herbicide-contaminated soil

A Gram-stain-negative, rod-shaped, non-motile, non-spore-forming bacterium, designated strain BY-1^T, was isolated from a soil sample from the city of Qiqihar in Heilongjiang Province, PR China. Strain BY-1^T grew optimally at pH 7.0 and 30–35 °C in the presence of 0.5 % (w/v) NaCl. Analysis of 16S rRNA gene sequences revealed that strain BY-1^T fell within the radiation of the genus Pseudomonas, and showed highest 16S rRNA gene sequence similarities to Pseudomonas luteola IAM 13000^T (99.5 %) and Pseudomonas duriflava HR2^T (97.3 %); the levels of sequence similarity with respect to other recognized species of the genus Pseudomonas were <96.7 %. Strain BY-1^T showed low DNA–DNA relatedness values with Pseudomonas luteola IAM 13000^T (29±3.1 %) and Pseudomonas duriflava HR2^T (21±1.5 %). The G+C content of the genomic DNA of strain BY-1^T was 55.3 mol%. The major fatty acids were C_18 : 1, C_16 : 0 and summed feature 3 (C_16 : 1ω6c and/or C_16 : 1ω7c). Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, and the major ubiquinone was Q-9. Data obtained in this study indicated that this isolate represents a novel species of the genus Pseudomonas, for which the name Pseudomonas zeshuii sp. nov. is proposed. The type strain is BY-1^T ( = KACC 15471^T = ACCC 05688^T).