Parabacteroides chartae sp. nov., an obligately anaerobic species from wastewater of a paper mill

A bacterial strain, designated NS31-3^T, was isolated from the wastewater of a paper mill. Cells of the isolate were obligately anaerobic, non-pigmented, non-motile, Gram-negative, short rods (0.7–1.0×1.4–2.5 µm). The isolate was able to grow on media containing 20 % bile salts. API 20A tests showed that acid was produced from glucose, lactose, sucrose, maltose, d-xylose, l-arabinose, cellobiose, d-mannose, d-melezitose, d-raffinose, d-trehalose, d-mannitol, salicin and d-sorbitol. The main fermentation products from PYG broth were lactic acid, propionic acid, formic acid and acetic acid. Chemotaxonomic analysis showed that the major fatty acids were anteiso-C_15 : 0, C_15 : 0 and iso-C_17 : 0 3-OH and the predominant respiratory quinones were MK-9 and MK-10. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain NS31-3^T was related to members of genus Parabacteroides (91.2–93.2 % sequence similarity); the isolate had the closest affinity with Parabacteroides merdae JCM 9497^T. The G+C content of the genomic DNA was 37.2 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic analysis, strain NS31-3^T represents a novel species of the genus Parabacteroides, for which the name Parabacteroides chartae sp. nov. is proposed. The type strain is NS31-3^T ( = JCM 17797^T  = DSM 24967^T).