SGM Journals
Actinobacteria

Amycolatopsis dongchuanensis sp. nov., an actinobacterium isolated from soil

Guo-Xing Nie, Hong Ming, Shuai Li, En-Min Zhou, Juan Cheng, Xia Tang, Hui-Gen Feng, Shu-Kun Tang, Wen-Jun Li

  • 1College of Life Sciences, Henan Normal University, Xinxiang 453007, PR China
  • 2Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China
  • 3Department of Life Sciences and Technology, Xinxiang Medical University, Xinxiang 453003, PR China
  • Correspondence
    Wen-Jun Li wjli{at}ynu.edu.cn Shu-Kun Tang tangshukun{at}hotmail.com

    • International Journal of Systematic and Evolutionary Microbiology 2012; 62(Pt 11):2650–2656 · https://doi.org/10.1099/ijs.0.038125-0

    View at publisher PubMed

    Abstract

    A novel actinomycete strain, designated YIM 75904T, was isolated from a soil sample that had been collected from a dry and hot river valley in Dongchuan county, Yunnan province, south-western China. The taxonomic position of the novel strain was investigated by a polyphasic approach. In phylogenetic analyses based on 16S rRNA gene sequences, strain YIM 75904T formed a distinct clade within the genus Amycolatopsis and appeared to be closely related to Amycolatopsis sacchari K24T (99.3 % sequence similarity). Strain YIM 75904T had a type-IV cell wall, with no detectable mycolic acids, and had MK-9(H4) as its predominant menaquonine. Its cell wall contained meso-diaminopimelic acid, galactose, glucose and arabinose, and its major cellular fatty acids were iso-C16 : 0, iso-C15 : 0, anteiso-C17 : 0 and anteiso-C15 : 0. The genomic DNA G+C content of the novel strain was 68.5 mol%. Based on the results of physiological and biochemical tests and DNA–DNA hybridizations, strain YIM 75904T represents a novel species of the genus Amycolatopsis for which the name Amycolatopsis dongchuanensis sp. nov. is proposed. The type strain is YIM 75904T ( = CCTCC AA 2011016T  = JCM 18054T).

    • ↵† These authors contributed equally to this work.

    • The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain YIM 75904T is JN656710.

    • Two supplementary figures and two supplementary tables are available with the online version of this paper.