Paracoccus rhizosphaerae sp. nov., isolated from the rhizosphere of the plant Crossostephium chinense (L.) Makino (Seremban)

P. Kämpfer, Wei-An Lai, A. B. Arun, Chiu-Chung Young, P. D. Rekha, K. Martin, H.-J. Busse, Wen-Ming Chen

¹Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany

²College of Agriculture and Natural Resources, Department of Soil & Environmental Sciences, National Chung Hsing University, Taichung, Taiwan, ROC

³Yenepoya Research Centre, Yenepoya University, University Road, Deralakatee, Mangalore, Karnataka, India

⁴Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie eV, Hans-Knöll-Institut, D-07745 Jena, Germany

⁵Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, A-1210 Wien, Austria

⁶Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, Kaohsiung, Taiwan, ROC

Correspondence
Peter Kämpfer peter.kaempfer{at}umwelt.uni-giessen.de

International Journal of Systematic and Evolutionary Microbiology 2012; 62(Pt 11):2750–2756 · https://doi.org/10.1099/ijs.0.039057-0

Abstract

A Gram-negative, coccoid-shaped bacterium, strain CC-CCM15-8^T, was isolated from a rhizosphere soil sample of the plant Crossostephium chinense (L.) Makino (Seremban) from Budai Township, Chiayi County, Taiwan. 16S rRNA gene sequence analysis clearly allocated strain CC-CCM15-8^T to the Paracoccus cluster, showing highest similarities to the type strains of ‘Paracoccus beibuensis’ (98.8 %), Paracoccus homiensis (97.6 %), Paracoccus aestuarii (97.7 %) and Paracoccus zeaxanthinifaciens (97.7 %). The fatty acid profile, comprising C_18 : 1ω7c as the major component and C_10 : 0 3-OH as the characteristic hydroxylated fatty acid, supported the placement of strain CC-CCM15-8^T within the genus Paracoccus. The polyamine pattern consisted of putrescine and spermidine as major components. Ubiqinone Q-10 was the major quinone type (95 %); ubiquinone Q-9 was also detected (5 %). The complex polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, and unidentified phospholipids, lipids and glycolipids. Levels of DNA–DNA relatedness between strain CC-CCM15-8^T and ‘P. beibuensis’ LMG 25871^T, P. aestuarii DSM 19484^T, P. zeaxanthinifaciens LMG 21993^T and P. homiensis KACC 11518^T were 24.9 % (34.8 %, reciprocal analysis), 15.7 % (17.5 %), 17.7 % (23.4 %) and 16.0 % (25.4 %), respectively. Physiological and biochemical test results allowed the phenotypic differentiation of strain CC-CCM15-8^T from its closest relatives in the genus Paracoccus. Based on the data presented, it is concluded that strain CC-CCM15-8^T represents a novel species of the genus Paracoccus, for which the name Paracoccus rhizosphaerae sp. nov. is proposed. The type strain is CC-CCM15-8^T ( = LMG 26205^T = CCM 7904^T).