Celerinatantimonas yamalensis sp. nov., a cold-adapted diazotrophic bacterium from a cold permafrost brine

Viktoria Shcherbakova, Nataliya Chuvilskaya, Elizaveta Rivkina, Nikita Demidov, Victoria Uchaeva, Stanislav Suetin, Nataliya Suzina, David Gilichinsky

¹All Russian Collection of Microorganisms (VKM), Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, Russian Federation, 142290

²Institute of Physicochemical and Biological Problems in Soil Sciences, Russian Academy of Sciences, Pushchino, Moscow Region, Russian Federation, 142290

³Vavilov Institute of General Genetics, Russian Academy of Sciences, Gubkin street 3, Moscow, Russian Federation, 119991

Correspondence
Viktoria Shcherbakova vshakola{at}gmail.com

International Journal of Systematic and Evolutionary Microbiology 2013; 63(Pt 12):4421–4427 · https://doi.org/10.1099/ijs.0.045997-0

Abstract

A facultatively anaerobic nitrogen-fixing bacterium, strain C7^T, was isolated from a permafrost cryopeg on the Yamal Peninsula, Russia. Comparative analysis of 16S rRNA gene sequences revealed that this bacterium was closely related to Celerinatantimonas diazotrophica S-G2-2^T with a similarity of 95.5 %. Strain C7^T differed from in its ability to hydrolyse gelatin and inability to use d-mannose, melibiose, l-rhamnose, myo-inositol, lactose, lactulose, d-mannitol, trehalose, dl-lactate, glycogen or l-proline as sole carbon sources. In addition, strain C7^T grew over a temperature range of 0–34 °C with optimum growth at 18–22 °C. The whole-cell fatty acid profile included C_16 : 0, C_16 : 1ω7, C_18 : 1ω7, C₁₇ cyclo and summed feature 2 [comprising C_12 : 0 aldehyde and/or unknown fatty acid 10.913 (MIDI designation) and/or iso-C_16 : 1/C_14 : 0 3-OH]. The DNA G+C content was 44.7 mol%. Strain C7^T is thus considered to represent a novel species, for which the name Celerinatantimonas yamalensis sp. nov. is proposed. The type strain is C7^T ( = VKM B-2511^T = DSM 21888^T).