SGM Journals
Proteobacteria

Alcanivorax marinus sp. nov., isolated from deep-sea water

Qiliang Lai, Jianning Wang, Li Gu, Tianling Zheng, Zongze Shao

  • 1MOE of Key Lab for Coast and Wetland Ecosystem, School of Life Sciences, Xiamen University, Xiamen 361005, People's Republic of China
  • 2State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA; Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen 361005, China
  • Correspondence
    Tianling Zheng wshwzh{at}xmu.edu.cn Zongze Shao shaozz{at}163.com

    • International Journal of Systematic and Evolutionary Microbiology 2013; 63(Pt 12):4428–4432 · https://doi.org/10.1099/ijs.0.049957-0

    View at publisher PubMed

    Abstract

    A taxonomic study was carried out on strain R8-12T, which was isolated from deep-sea water of the Indian Ocean during the screening of oil-degrading bacteria. The isolate was Gram-stain-negative, oxidase and catalase-positive. Growth was observed at salinities from 0.5 to 15 % (optimum 3 %), at pH from 6–10 (optimum 7–8) and at temperatures from 10 to 42 °C (optimum 28 °C). On the basis of 16S rRNA gene sequence similarity, strain R8-12T was shown to belong to the genus Alcanivorax and to be related to Alcanivorax venustensis DSM 13974T (97.2 %), A. dieselolei B-5T (95.0 %), A. balearicus MACL04T (94.6 %), A. hongdengensis A-11-3T (94.3 %), A. jadensis T9T (93.8 %), A. borkumensis SK2T (93.7 %) and A. pacificus W11-5T (93.7 %). The gyrB sequence similarities between R8-12T and other species of the genus Alcanivorax ranged from 77.9 % to 86.9 %. The major fatty acids were C16 : 0 (31.8 %), C18 : 1ω7c (20.3 %), C19 : 0ω8c cyclo (15.8 %) and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c) (8.9 %). The polar lipids were phosphatidylglycerol (PG), phosphatidylethanolamine (PE), two aminolipids (AL1–AL2) and two phospholipids (PL1–PL2). Three alkane hydroxylase (alkB) genes were identified in the genome. The G+C content of the chromosomal DNA was 66.1 mol%. DNA–DNA hybridization showed that strain R8-12T and A. venustensis DSM 13974T had a DNA–DNA relatedness of 63±3 %. According to its phenotypic features and fatty acid composition as well as the 16S rRNA and gyrB gene sequences, the novel strain represents a member of the genus Alcanivorax, but could be easily distinguished from all other known species of the genus Alcanivorax described to date. The name Alcanivorax marinus sp. nov. is proposed, with the type strain R8-12T ( = MCCC 1A00382T = LMG 24621T = CCTCC AB 208234T).

    • The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA, gyrB and partial alkB gene (alkB1, alkB2 and alkB3) sequences of Alcanivorax marinus R8-12T are KC415169, KC415173 and KC415170–KC415172, respectively.

    • Four supplementary figures and two supplementary tables are available with the online version of this paper.