SGM Journals
Proteobacteria

Ciceribacter lividus gen. nov., sp. nov., isolated from rhizosphere soil of chick pea (Cicer arietinum L.)

R. Kathiravan, S. Jegan, V. Ganga, V. R. Prabavathy, L. Tushar, Ch. Sasikala, Ch. V. Ramana

  • 1Microbiology Department, M. S. Swaminathan Research Foundation, 3rd Cross street, Taramani institutional area, Chennai 600113, India
  • 2Department of Plant Sciences, School of Life Sciences, University of Hyderabad, PO Central University, Gachibowli, Hyderabad 500046, India
  • 3Bacterial Discovery laboratory, Centre for Environment, Institute of Science and Technology, JNT University Hyderabad, Kukatpally, Hyderabad 500085, India
  • Correspondence
    V. R. Prabavathy prabavathyvr{at}mssrf.res.in or Microbiology{at}mssrf.res.in

    • International Journal of Systematic and Evolutionary Microbiology 2013; 63(Pt 12):4484–4488 · https://doi.org/10.1099/ijs.0.049726-0

    View at publisher PubMed

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    Abstract

    The taxonomic position of strain MSSRFBL1T, isolated from chickpea rhizosphere soil from Kannivadi, India, was determined. Strain MSSRFBL1T formed bluish black colonies, stained Gram-negative and was motile, aerobic, capable of fixing dinitrogen, oxidase-negative and catalase-positive. Q-10 was the major respiratory quinone. Major fatty acids of strain MSSRFBL1T were C18 : 1ω7c and C19 : 0cycloω8c. Minor amounts of C18 : 0, C12 : 0, C14 : 0 3-OH, C18 : 0 3-OH, C16 : 0, C16 : 1ω6c/C16 : 1ω7c, C17 : 0 3-OH and C20 : 1ω7c were also present. Polar lipids included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylcholine and two unidentified glycolipids. Bacteriohopane derivatives (BHD1 and 2), diplopterol, diploptene, bishomohopanediol, adenosylhopane and 2β-methyl bacteriohopanetetrol were the major hopanoids of strain MSSRFBL1T. The genomic DNA G+C content was 71 mol%. EzTaxon-e-based blast analysis of the 16S rRNA gene indicated the highest similarity of strain MSSRFBL1T to Ensifer adhaerens LMG 20216T (97.3 %) and other members of the genus Ensifer (<96.9 %) in the family Rhizobiaceae of the class Alphaproteobacteria. However, phylogenetic analysis based on 16S rRNA, recA, thrC and dnaK gene sequences showed distinct out-grouping from the recognized genera of the family Rhizobiaceae. Based on phenotypic, genotypic and chemotaxonomic characters, strain MSSRFBL1T represents a novel species in a new genus in the family Rhizobiaceae for which the name Ciceribacter lividus gen. nov., sp. nov. is proposed. The type strain of Ciceribacter lividus is MSSRFBL1T ( = DSM 25528T = KCTC 32403T).

    • The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA, recA, dnaK, thrC and nifH gene sequences of strain MSSRFBL1T are JQ230000, KC189949, KC189950, KC189951 and KC189952, respectively.

    • Five supplementary figures are available with the online version of this paper.