SGM Journals
Actinobacteria

Corynebacterium frankenforstense sp. nov. and Corynebacterium lactis sp. nov., isolated from raw cow milk

Raika Wiertz, Stefanie Christine Schulz, Ute Müller, Peter Kämpfer, André Lipski

  • 1Rheinische Friedrich-Wilhelms-Universität Bonn, Institut für Ernährungs- und Lebensmittelwissenschaften, Abteilung Lebensmittelmikrobiologie und -hygiene, Meckenheimer Allee 168, 53115 Bonn, Germany
  • 2Rheinische Friedrich-Wilhelms-Universität Bonn, Institut für Tierwissenschaften, Abteilung Physiologie und Hygiene, Katzenburgweg 7-9, 53115 Bonn, Germany
  • 3Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, 35392 Giessen, Germany
  • Correspondence
    André Lipski lipski{at}uni-bonn.de

    • International Journal of Systematic and Evolutionary Microbiology 2013; 63(Pt 12):4495–4501 · https://doi.org/10.1099/ijs.0.050757-0

    View at publisher PubMed

    Abstract

    Two groups of Gram-stain positive, aerobic bacterial strains were isolated from raw cow’s milk, from a milking machine and from bulk tank milk. Based on their 16S rRNA gene sequences these isolates formed two distinct groups within the genus Corynebacterium. The sequence similarities of the isolates to the type strains of species of the genus Corynebacterium were below 98.4 %. The presence of menaquinones MK-8(H2) and MK-9(H2), the predominant fatty acid 18 : 1 cis 9 and a polar lipid pattern with several phospholipids but without aminolipids was in accord with the characteristics of this genus. The results of DNA–DNA hybridization, biochemical tests and chemotaxonomic properties allowed genotypic and phenotypic differentiation of the strains from all known species of the genus Corynebacterium. Therefore, the isolates were assigned to two novel species of this genus for which the names Corynebacterium frankenforstense sp. nov. (type strain ST18T = DSM 45800T = CCUG 63371T), and Corynebacterium lactis sp. nov. (type strain RW2-5T = DSM 45799T = CCUG 63372T) are proposed, respectively.

    • The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains ST18T, RW2-45.1, RW2-5T and RW3-42 are HE983827, HE983828, HE983829 and HE983830, respectively.

    • Three supplementary tables and two supplementary figures are available with the online version of this paper.