Enterococcus diestrammenae sp. nov., isolated from the gut of Diestrammena coreana

A novel Gram-stain-positive, facultatively anaerobic, non-motile and lactic-acid-producing bacterium, designated strain ORL-24^T, was isolated from the gut of the camel cricket, Diestrammena coreana. Optimal growth occurred at 37 °C, pH 8 and with 0 % (w/v) NaCl. The ratio of l-lactate to d-lactate in strain ORL-24^T was 96 : 4. Lancefield antigen D was not detected. The strain was negative for oxidase activity and catalase activity. According to a phylogenetic analysis based on 16S rRNA gene sequences, strain ORL-24^T was most closely related to the type strain of Enterococcus asini (96.9 % similarity). Comparative pheS and rpoA sequence analyses of strain ORL-24^T indicated that the strain belonged to the genus Enterococcus. The major fatty acids were C_16 : 0 and C_18 : 1ω9c. The DNA G+C content was 41.3 mol%. Based on phenotypic, genotypic and phylogenetic analyses, strain ORL-24^T represents a novel species of the genus Enterococcus, for which the name Enterococcus diestrammenae is proposed. The type strain is ORL-24^T ( = KACC 16708^T = JCM 18359^T).