SGM Journals
Actinobacteria

Micromonospora kangleipakensis sp. nov., isolated from a sample of limestone quarry

Salam Nimaichand, Yong-Guang Zhang, Juan Cheng, Li Li, Dao-Feng Zhang, En-Min Zhou, Lei Dong, Debananda S. Ningthoujam, Wen-Jun Li

  • 1Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürűmqi 830011, PR China
  • 2Microbial Biotechnology Research Laboratory, Department of Biochemistry, Manipur University, Canchipur, Imphal, 795003, Manipur, India
  • 3Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, PR China
  • Correspondence
    Debananda S. Ningthoujam debananda.ningthoujam{at}gmail.com Wen-Jun Li liact{at}hotmail.com

    • International Journal of Systematic and Evolutionary Microbiology 2013; 63(Pt 12):4546–4551 · https://doi.org/10.1099/ijs.0.052746-0

    View at publisher PubMed

    Abstract

    Strain MBRL 34T, isolated from a sample of limestone quarry located at Hundung, Manipur, India, was characterized by polyphasic taxonomy. The strain showed the highest 16S rRNA gene sequence similarity with Micromonospora echinaurantica DSM 43904T (98.4 %), but formed a monophyletic clade with Micromonospora coerulea DSM 43143T (98.3 %) in the neighbour-joining tree. DNA–DNA hybridization experiments gave a DNA–DNA relatedness value of 53.1 % between MBRL 34T and M. coerulea DSM 43143T. Strain MBRL 34T contained meso-diaminopimelic acid, galactose and glucose in the whole-cell hydrolysates along with small amounts of mannose, xylose, rhamnose and ribose. The major polar lipids detected were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositolmannoside, along with an unknown lipid. MK-10(H6), MK-10(H2) MK-11(H4) and MK-10(H4) were the predominant menaquinones detected. The major fatty acids were iso-C16 : 0 and iso-C15 : 0. The G+C content of the genomic DNA was 73.5 %. Based on the taxonomic characteristics from a polyphasic study, strain MBRL 34T merits recognition as a representative of a novel species of the genus Micromonospora for which the name Micromonospora kangleipakensis sp. nov. is proposed; the type strain is MBRL 34T ( = DSM 45612T = JCM 17696T).

    • The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain MBRL 34T is JN560152.

    • A supplementary figure and supplementary table are available with the online version of this paper.